Optimization of Alginate Production by Azotobacter vinelandii Isolated from agriculture Soil
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Abstract
Alginate, a biopolymer with various applications in industry and medicine, can be produced by Azotobacter. These bacteria are often present in soil samples and provide nitrogen fixation. This study is designed to isolate and identify a strain with high alginate production efficiency from soil samples and optimize production environmental conditions. Eighty soil samples were collected from 5 areas, and after cultivation on Mannitol agar and Ashby’s Mannitol agar, phenotypic identification was performed based on the morphology of the colonies. Identification of the superior strain in alginate production conducted by carbazole agent test. Then three carbon sources of sucrose, mannitol, and glucose, and three nitrogen sources, including peptone, yeast extract, and malt extracted of different pH, were used to identify the optimal conditions for alginate production. The results of 16s rRNA sequencing showed Azotobacter vinelandii as the superior strain. Also, under optimal conditions, which include sucrose, peptone, pH seven, and a temperature of 30 °C, the production of alginate by this strain was 1.65 g / L. Similar to other studies, it found a significant relationship between the presence of a suitable carbon source (sucrose), temperature, and optimal pH with increased growth and, ultimately, increased alginate production.
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